Step 1: Manipulations within the 7700 software:

1. Open the SDS data file to be analyzed.
2. Analyze the data and set the baseline and threshold values according to Applied Biosystems guidelines. To obtain more information on baseline and thresholdhold setting guidelines please refer to pages 23-32 in the TaqMan Cytokine Gene Expression Plate I Protocol, available on-line at: http://docs.appliedbiosystems.com/pebiodocs/04306744.pdf
3. From the File menu, select "Export" and "Clipped data". You may either export all of the data or data from selected wells (click the appropriate button).
4. The default file name will be "data.clipped". Provide a name and file location for this data. It is suggested that you keep the suffix .clipped.

Step 2: Open a new Workbook in Microsoft Excel:

Select "File", "Open". Open the .clipped file.

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Create a chart by either selecting "Chart" from the "Insert" tab, or by clicking the Chart Wizard button. Select XY (Scatter) as the chart type, and select the Chart sub-type shown. Click "Next".

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