Tm Calculator

 
Directions:

To calculate the optimal melting temperatures of your primers, enter each primer's concentration, its 5'-3' sequence, and the salt concentration (K+ and Na+) of your PCR or Real-Time PCR reaction.
Note: If entering info only for Primer #1, ignore result values given for Primer #2.

 
  
Primer #1
Salt Concentration (mM)  Primer Concentration (µM) 
5' 3'
 
Primer #2
Salt Concentration (mM)  Primer Concentration (µM) 
5' 3'

 
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