Applied Biosystems 3730xl DNA Analyzer

Specifications


Sequencing Production Capacity

Run Module Capillary Length to Detector (cm) Runs/ Day LOR* Phred Q20 bases/read Phred Q20 bases/day
Rapid 36 40 550 500 1,920,000
Standard 36 24 700 650 1,497,600
Long-Read 50 12 > 1,000 > 800 > 921,600
* 98.5% basecalling accuracy, less than 2% N's, using pGEM-3Zf(+) as template.

Capillary Arrays and Separation Matrix

Capillary Separation Distance (cm) Dimensions Polymer Consumed/run
36 150 μm o.d./50 μm i.d. Approx. 200 μL
50 150 μm o.d./50 μm i.d. Approx. 250 μL

Reagents
  • BigDye® Terminator v 1.1
  • BigDye® Terminator v 3.0
  • BigDye® Terminator v 3.1
Instrument configuration
  • CE Instrument
  • Computer and Flat Panel Monitor
  • Installation Chemistry and Accessories
  • Collection and Analysis Software
Computer Specifications
  • Base Unit: Pentium® IV Processor 2.00 GHz/400 MHz
  • Memory: 1 GB, PC 800 @ 400 MHz
  • Hard Drive: 72 GB
  • Operating System: Windows® 2000 Professional Edition
  • Monitor: 17" Flat Panel
  • DVD-ROM Drive
Integrated Plate Stacker
  • Houses 16 sample plates at any time
  • Accommodates 96-well and 384-well plates
  • Accessible any time except when autosampler is moving
Sample Volumes
  • For 384-Well Sample Plates: 5-30 μL
  • For 96-Well Sample Plates: 10-50 μL
Plate Seal
  • Septa
  • Polypropylene heat seal (automatic onboard piercing)
Laser

Argon-ion multi-line, single mode laser: primary excitation lines 488 and 514.5 nm.

Operating Environment
  • Ambient temperature: 18�C to 30�C
  • Humidity: 20 to 80% (non-condensing)
Oven Temperature

Active temperature control between 18˚C to 70˚C

Electrical
  • Main Power: 200-220 V or 230-240 V+/-10%, 50/60 Hz
  • Power Rating: Maximum input of 2500 VA
  • Circuit Current: Maximum of 15 amps. The instrument requires a 30 amp receptacle to match one of the two power cord configurations that ship with the system. The electrical receptacle must be located within 3 m (10 ft.) of the back of the instrument. Do not use extension chords.
  • Maximum Power Dissipation: 2,000 watts (approximation)
Dimensions (electrophoresis unit)
  • Width: 100 cm
  • Depth: 73 cm
  • Height: 89 cm
  • Weight: approximately 180 kg

Windows is a registered trademark of Microsoft Corporation.
Pentium is a registered trademark of Intel Corporation

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